Background: The operating room staff is exposed to stress due to the frequent contacts with patients, which may ultimately leads to burnout. The aim of this study was to determine burnout and its related factors among the operating room staff in teaching hospitals affiliated with the Isfahan University of Medical Sciencesin 2018.
Methods: The present quantitative and qualitative study was conducted in Isfahan. In the quantitative phase, 206 employees were selected by simple random sampling method and data were analyzed by SPSS₂₃ using descriptive statistics, one-way ANOVA, t-test, and other related tests.  Data were collected by “Demographic form” and “Maslach Burnout Inventory” (MBI) questionnaires.  In the qualitative phase, data were collected by conducting interviews with 20 participants including managers, supervisors, and matrons of the operating roomby using MAXQDA12. Software.
Results: Results showed that the demographic variables had a significant relationship with burnout variable and its dimensions. Work experience and academic degree had a significant relationship with depersonalization (P-value < 0.05). According to the multiple linear regression, academic degree had the highest predictive power for depersonalization. In the qualitative phase, three factors associated with burnout included: internal factors of organization, external factors of organization and individual and personal factors of organization.
Conclusion: Burnout affects the staff's efficiency and performance in the operating room. Therefore, managers must provide programs to reduce burnout among the staff, so that they can provide high-quality services and reduce costs.